In vitro Conservation and Exploiting Polyembryonate Potential of Synthetic Seeds of Malaxis acuminata D. Don

Synthetic seeds were prepared in Malaxis acuminata by encapsulating shoot tip derived protocorm-like bodies (PLBs; 0.2-0.5 cm) a hydrated gel capsule composed of calcium alginate matrix (sodium alginate; 3.5% and chloride; 75 mM) liquid Murashige Skoog, 1962 (MS) medium. varied physical features with the concentration gelling agent chloride. In vitro germination, optimum response was achieved MS medium fortified BAP (1mgl-1); where synthetic germinated 100% frequency. Physically firm, globular, non-leaky, self-breaking beads efficiently which could be stored for up to 90 days at 4℃. At room temperature, 50% frequency after 15 germination steadily reduced 0% 45 storage. Under vivo conditions, showed 80% bead-to-plant conversion supplemented antibacterial, antifungal agents (1mg/I). Plant Tissue Cult. & Biotech. 33(1): 9-15, 2023 (June)